Abstract: Soybean isoflavones is a class of secondary metabolites that contribute to human health. Soybean chalcone reductase（CHR） gene is one of the key enzymes controlling the biosynthesis of daidzein, the main component of soybean isoflavones. In order to broaden the research types of soybean CHR, promote the research of isoflavone metabolism mechanism, and then promote the improvement of soybean varieties, this study used genetic engineering technology to perform gene cloning and bioinformatics analysis of GmCHR2-1.And then we constructed a recombinant plant expression vector pCAMBIA3301-CHR2-1, transformed the vector into the Dongnong 50 soybean receptor through agrobacterium-mediated method, and identified regenerated soybean transformed plants with PCR screening. The results showed that the protein encoded by the GmCHR2-1 gene is a non-secretory protein and may play a major role in the cytoplasm. The protein is a non-transmembrane protein and does not migrate in the cell. The protein has 22 potential phosphorylation sites, including four threonine（Thr）, and eighteen serine（Ser） composed of 40.63% α-helix, 14.29% extended chain, 4.13% β-turn and 40.95% random curl. PCR identified that the genome of the transformed plant contained the glufosinate resistance gene Bar, the terminator NOS and the promoter 35 S site, and it was preliminarily determined that GmCHR2-1 was transferred into the genome of Dongnong 50 soybean variety, and we finally obtained 12 T₂ positive plants.