Application Progress of PCR Technology in Transgenic Detection of Soybean Products

In recent years, the application rate of genetically modified soybeans in the world has reached about 78%. Genetically modified（GM） soybeans are mainly used for soybean oil production, and their products have been more and more widely involved in consumers′ dietary life. The quantity of Chinese self-produced soybeans is less than one-fifth of the quantity of imported soybeans, and the imported soybeans are mainly GM soybeans. In order to standardize the circulation, processing and consumption of GM soybeans, China adopts mandatory labeling management for GM soybean products, which puts forward higher requirements for the detection technology of GM products. At present, the most commonly used detection method is nucleic acid amplification detection technology, of which the polymerase chain reaction（PCR） technology is the most widely used, mainly including ordinary qualitative PCR, nested PCR, real-time fluorescent quantitative PCR, multiplex PCR, digital PCR, etc. This paper reviewed the application of different PCR technologies in the detection of GM soybean products, and compared their advantages and disadvantages. The applicability of PCR technologies to GM soybean products with different processing degrees was explored, and the future development direction of PCR technology for GM soybean products was prospected. This paper aims to provide a theoretical reference for the selection of the most suitable PCR technology in the detection of GM soybean products, and to help promote the implementation of the biosafety management of GM soybean products in China.