ldentification of the Viral Pathogens Infecting Soybean in Jining,Shandong Province

Soybean virus disease is a devastating disease occurring in soybean production areas all over the world. In order to clarify the type and prevalence of soybean virus disease in the Jining test field in Shandong, we used the dot enzyme linked immunosorbent assay（Dot-blot ELISA） to identify SMV,and reverse transcription PCR（RT-PCR） to identify 14 kinds of viruses in 37 samples collected from this area. It was found that among all samples, 22 samples were detected as soybean mosaic virus（SMV）, with a detection rate of 51.3%, 18 samples as bean common mosaic virus（BCMV）, with a detection rate of 32.4%. Among the 23 samples with virus detected, six were compound infections of SMV and BCMV with compound infection rate of 24.3%. This is the first time that BCMV infection has been detected in soybean planting areas in Jining, Shandong Province, providing a direction for local soybean virus disease prevention, control and disease-resistant breeding.