Determination of Active Ingredients of Sojae Semen Praeparatum by Multi-Components with Single Marker

In order to quickly determine the content of the main active ingredients in the Sojae Semen and evaluate its quality, this study used 28 batches of Sojae Semen Praeparatum from different origins as the test materials.A quantitative analysis of multi-components with single marker（QAMS） was established for the simultaneous determination of daidzin, glycitin, genistin, daidzein, glycitein, genistein in crude and processed Sojae Semen Praeparatum, which was proved to be a accurate and feasible method in the quality analysis in Sojae Semen Praeparatum.The results showed that the established relative correction factors of daidzin, glycitin, daidzein, glycitein, genistein were 0.666,1.089,0.926,0.978 and 1.894,with 70% methanol as the extraction solvent, heating and refluxing extraction for 60 min for pretreatment, and using genistin as the internal reference by using HPLC.The reproducibility of the relative correction factor was good, and there was no significant difference between the QAMS method and external standard measurement value.This method is accurate and feasible, and can be used as quality control of Sojae Semen.