Abstract: 【Objective】 In order to improve the survival rate of Salmon trutta fingerlings in scientific research sampling, production selection and breeding and release marking.【Method】 MS-222 and eugenol were used to compare the anesthetic effects of the fingerling with body mass（143.2±7.44） g test.【Result】 When the mass concentrations of MS-222 and clove oil reached 100～160 and 50～110 mg/L respectively, the degree of anesthesia reached the stage of medulla oblongata anesthesia（A6）.With the increase of the concentrations of MS-222 and Eugenol, the anesthesia time decreased and the resuscitation time increased The survival rate was 100% at 24 h, MS-222 at the concentration of 130～140 mg/L was anesthetized within 3 minutes and resuscitated within 5 minutes.MS-222 and eugenol showed a decreasing trend of anesthesia and recovery time with the increase of water temperature.The survival rate of MS-222 was 66.7% when the time of MS-222 was 25 min, and the recovery time of eugenol was decreasing at first and then increasing with the time of air exposure, the survival rate decreased to 60% and 20% after 20 and 25 min air exposure respectively.【Conglusion】 MS-222 is a better anesthetic agent than eugenol for Salmon trutta. It was suggested that MS-222 with 25 mg/L shallow anesthetic concentration and 130 mg/L deep anesthetic concentration should be used in practice. The optimal anesthetic temperature was 12～16 ℃,the time of air exposure was less than 20 min.