Abstract: 【Objective】 To investigate the antibiotic resistance of Enterococcus from different livestock in parts of Gansu Province.【Method】 Enterococcus was isolated from 300 faecal samples and further identified by differential medium and PCR,the K-B method was used to determine the sensitivity of 12 antimicrobial agents against Enterococcus. The broth microdilution method was used to determine the minimum inhibitory concentration（MIC） of vancomycin against Enterococcus,and vancomycin resistance genes were detected by PCR.【Result】 A total of 246 Enterococcus strains were isolated from 300 samples,the resistance rates of oxacillin,TMPco and clindamycin were the highest with rates,and multidrug-resistant Enterococcus accounted for 82. 11%. There were significant differences among drug resistance of Enterococcus from different animals,and multidrug-resistant Enterococcus from pig accounted for 94. 4%. There were significant differences between the drug resistance of different Enterococcus,and multidrug-resistant E. faecium accounted for 100%. The van C1 and/or van C2/3 genes were carried by Enterococcus,and the carrying rate of the vancomycin resistance genes was 24. 39%.【Conclusion】 The Enterococcus from animals had high drug resistance to antibiotics,and with the attribute of multidrug resistance and the risk of vancomycin resistance transfer in parts of Gansu Province.