Abstract: 【Objective】 To study the biological function of the vernalization-related SOSEKI（SOK）gene in Angelica sinensis and to analyze its expression pattern in response to vernalization and freezing temperatures.【Method】 The vernalization-related FLC gene was found out based on the full-length transcriptome sequencing of A. sinensis seedlings stored at vernalization（0 ℃） and freezing（-3 ℃） temperatures,its biological function was analyzed using the online bioinformatics tools, and gene expression was validated by qRT-PCR.【Result】 A three-gene cluster AsSOK of 1 005 bases was identified in A. sinensis,consisting of FLC and its two flanking genes UPSTREAM OF FLC（UFC） and DOWNSTREAM OF FLC（DFC）,and localized subcellularly in nucleus. Conserved motif and phylogenetic analysis showed that the AsSOK protein contained four motifs（motif-2,-4,-5, and-6） and had the closest relationship with LOC Dc108213152 of Daucus carota subsp. sativus with similar motif composition and position. Gene expression analysis showed that the expression level of AsSOK gene significantly decreased during vernalization,while it increased at freezing temperatures.【Conclusion】 This study is the first time to unravel and comprehensively analyze the biological function of vernalization-related AsSOK gene cluster, which will provide fundamental basis for regulating the bolting and flowering of A. sinensis by gene AsSOK editing technique.