Abstract: 【Objective】 To study the physiological response characteristics and salt tolerance differences of 13 wine grape cultivars at the seedling stage under salt stress.【Method】 In this experiment,13 kinds of annual grape cuttings were used as test materials. In the pot culture method,100,150,200 mmol/L NaCl solutions were irrigated in the matrix（nutrient soil∶vermiculite=1∶1） to simulate salt stress.【Result】 Compared to the control,the relative chlorophyll content（SPAD value） and the maximum photochemical efficiency（F_v/F_m） of 13 grape cultivars decreased with increasing salinity treatment concentration after 15 days of treatment with different concentrations of NaCl. The relative conductivity,the content of malondialdehyde（MDA）,soluble sugar,soluble protein,proline（Pro） and the activities of superoxide dismutase（SOD）,peroxidase（POD） and catalase（CAT） increased and increased first and then decreased with the increase of salt treatment concentration. Among them,under the treatment of 150 mmol/L NaCl,the evaluation indices of salt tolerance of 13 cultivars were significantly different from the control. The comprehensive analysis of the membership function showed that the salt tolerance of the varieties tested in this study was Pinot Noir＞Riesling＞Pinot Blanc＞Cabernet Gernischt＞Merlot＞Cabernet Ruby＞Cabernet Sauvignon＞Ugni Blanc＞Chardonnay＞Saperavi＞Cabernet Franc＞Syrah＞Italian Riesling. 【Conclusion】 13 grape varieties showed stress response after treatment with 100 mmol/L NaCl for 15 days. Of the 13 varieties tested in this study,Pinot Noir has the highest salt tolerance and Italian Riesling has the lowest salt tolerance.