高级检索+

单宁酸对豌豆蚜细胞色素P450和谷胱甘肽S-转移酶基因表达的影响

Effect of tannic acid on cytochrome P450 and glutathione S-transferase gene expression in Acyrthosiphon pisum

    摘要
  • 摘要: 【目的】明确豌豆蚜（Acyrthosiphon pisum）细胞色素P450酶（CYP450）和谷胱甘肽S-转移酶（GST）基因在转录水平对植物次生代谢物质单宁酸的响应，为探索CYP450基因和GST基因在豌豆蚜代谢单宁酸中的功能提供前期基础。【方法】采用荧光定量PCR技术，分析了单宁酸处理下豌豆蚜CYP450基因和GST基因的转录变化。【结果】经单宁酸处理后，豌豆蚜多个CYP450基因和GST基因的转录水平都表现出显著上调。其中，CYP450基因CYP4C107、CYP6K109、CYP6A1302、CYP303A1和CYP3077A1分别显著上调1.88、1.67、2.37、2.81和4.46倍；GST基因GST104、GST105、GST108和GST109分别显著上调1.42、2.07、4.89和1.41倍，GSTx1、GSTx4和GSTx6分别显著上调1.77、1.33和1.62倍，GSTm、GSTt、GSTcd和GSTd702分别显著上调1.60、1.44、1.40和4.27倍。【结论】CYP450基因和GST基因可能在豌豆蚜代谢单宁酸中发挥重要的作用。

     

    Abstract: 【Objective】 To determine the response of detoxification enzyme genes in A. pisum to tannic acid,which is a plant secondary metabolite,to provide some data to explore the functions of CYP450 and GST genes in tannic acid metabolism in A. pisum.【Method】 The cytochrome P450（CYP450） and glutathione S-transferases（GSTs） genes in pea aphids were measured by RT-qPCR after feeding on Vicia faba treated with tannic acid.【Result】 The expression of CYP450 and GST genes in A. pisum was significantly up-regulated after treatment with tannic acid. CYP4C107,CYP6K109,CYP6A1302,CYP303A1 and CYP3077A1 were dramatically up-regulated by 1. 88-,1. 67-,2. 37-,2. 81-and 4. 46-fold,respectively.In addition,GST104,GST105,GST108 and GST109 were up-regulated 1. 42-,2. 07-,4. 89-and 1. 41-fold,respectively. In addition,GSTx1,GSTx4 and GSTx6 were up-regulated 1. 77-,1. 33-and 1. 62-fold,respectively. GSTm,GSTt,GSTcd and GSTd702 were up-regulated 1. 60-,1. 44-,1. 40-and 4. 27-fold,respectively.【Conclusion】 The CYP450 and GST genes may play an important role in the metabolism of tannic acid in A. pisum.

     

    • HTML全文
    • 参考文献(38)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回