Abstract: 【Objective】 The objective of this study was to investigate the genetic diversity of plant germplasm resources,with a particular focus on Halogeton glomeratus. Additionally,we aimed to predict the genome size of H. glomeratus using flow cytometry,providing valuable insights for genome size determination in other halophyte species.【Method】 Halogeton glomeratus and Halogeton arachnoideus were selected as materials to establish a theoretical basis for species identification and explore the genetic relationships among halophytes. Chromosome karyotypes were analyzed using the conventional pressing method on root tip cells of H. glomeratus and H. arachnoideus. Furthermore,the genome size of H. glomeratus was predicted through flow cytometry.【Result】 Both H. glomeratus and H. arachnoideus were found to be diploid with a chromosome count of 2n=18. The karyotype formulas for the two cultivars were as follows:H. glomeratus—2 n=2 x=18=12 m+6 sm,H. arachnoideus-2 n=2 x=18=16 m+2 sm. The asymmetrical karyotype coefficients（AS. K%） were 61. 91% and 41. 91%,respectively. The karyotype symmetries were classified as 2A and 1B types. The estimated genome size of H. glomeratus was approximately（0. 95±0. 02）G.【Conclusion】 Among the two halophyte varieties studied,H. glomeratus displayed a higher level of evolutionary advancement compared to H. arachnoideus,which appeared to be a relatively original variety. A reliable method for determining the genome size of H. glomeratus using flow cytometry was established,and the genome size of H. glomeratus was successfully predicted. These findings contribute to the understanding of genetic diversity in halophytes and can serve as a reference for genome size determination in other species of halophytes.