Abstract: 【Objective】 To study the effects of polyethylene nano-packaging on the quality characteristics and nutritional components of Flammulina velutipes during storage.【Method】 A white strain of F. velutipes was used as the test material. The stain was packaged in polyethylene（PE） nano-film. The unpackaged CK group and the PE film packaged group were used as double controls. The storage experiment was carried out for 18 days at 4 ℃. Samples were taken every 3 days and the main quality indicators（weight loss,elongation and whiteness of the stalk） and nutritional components（total sugars,protein,vitamin C,crude polysaccharides,flavonoids and polyphenols） were determined dynamically.【Result】 During storage,the weight loss and stalk elongation of F. velutipes in the nano-film packaging group were significantly lower than those in the control group,the whiteness was significantly higher than that in the control group（P＜0. 05）,and the retention amount of nutrients was significantly higher than that in the control group（P＜0. 05）. After 18 days of storage,the contents of total sugars,protein,vitamin C,crude polysaccharides,flavonoids and total phenolics in F. velutipes were significantly higher than those in the PE packaging group by 14. 6%,20. 2%,20. 5%,9. 7%,8. 3% and 29. 9%,respectively（P＜0. 05）.【Conclusion】 Nano-packaging could effectively delay the quality deterioration and nutritional loss such as weight loss,stalk elongation and browning of F. velutipes during storage and was of great importance in maintaining the quality characteristics and nutritional content of F. velutipes.