Abstract: 【Objective】 The study aimed to explore the effect of isoflavones from Minshan red clover on the absorption and metabolism of calcium and phosphorus in animals and the underlying mechanisms.【Method】 We selected 80 Sprague-Dawley female rats（50～60 days old） and divided them into 8 groups（10rats in each group） randomly. Among them,1-7 groups were ovariectomized and intragastrically administrated with red clover isoflavones,the doses of which were 320,160,80,40,20,10 and 0 mg/（kg·d）,respectively,and the group 8 was a sham operation group,which was perfused with the same amount of normal saline for 60 days.【Result】 The doses of 40～320 mg/（kg·d） could increase the serum calcium content of the ovariectomized rats,and the tibial ash content,tibial calcium content and calcium phosphorus ratio of rats increased with the dose increasing. The dose of 20～40 mg/（kg·d） could up-regulate the expression of VDR mRNA and CaBP-9K mRNA in small intestine of rats and promoted the absorption of calcium in small intestine,and the doses of 160～320 mg/（kg·d） could upregulate the expression of VDR mRNA in rat kidney and promote the reabsorption of calcium and phosphorus in renal tubules.【Conclusion】 The isoflavones from Minshan red clover can promote calcium absorption in small intestine,reabsorption in kidney,and bone growth and mineralization of the ovariectomized rats.