Abstract:
【Objective】 The aim of this study was to establish large-scale artificial breeding technology and provide a scientific basis for the establishment of productive seedling and the protection of germplasm resources for Rhinogobio nasutus.【Method】 Fertilized eggs of Rhinogobio nasutus were obtained through artificially-induced spawning,and the morphological characteristics of embryo and larval development were recorded and analyzed at a constant water temperature of(23. 2±1. 0) °C.【Result】 The unfertilized mature eggs were spherical,light green,and drifting,with a diameter of(0. 60±0. 08) mm. After fertilization and hydration,the fully swollen eggs became translucent,with a diameter of(0. 76±0. 08) mm. Under the conditions of water temperature(23. 2±1. 0) °C and pH 8. 71~8. 86,the fertilized eggs developed into the blastula stage in 3. 67 hours,the gastrula stage in 5. 95 hours,embryo body formation in 9. 55 hours,organ formation in 13. 38 hours,muscle effect in 19. 98 hours,and heartbeat in 24. 05 hours. After 40. 43 hours,embryo development was completed,and the larval stage was reached. The overall length of newly hatched larvae ranged from about 3. 00 to 4. 00 mm,with a heartbeat of 101~132 beats per minute. The yolk of the larvae was completely absorbed within 2 days,and in some larvae,the cloaca was opened. All larvae started ingestion 3 days after hatching,under the condition of a water temperature of 26 ℃.【Conclusion】 This study recorded,analyzed,and described the process of embryonic development of the big-nosed minnow using artificial labor induction. Hatching larvae of Rhinogobio nasutus were successfully obtained. The embryonic development process went through seven stages,including the embryo dish stage,cleavage stage,blastocyst stage,gastrula stage,neuroembryonic stage,organ formation stage,and hatching out of the membrane. Each development stage consisted of several phases.