高级检索+

KNO3对NaCl胁迫下苹果砧木T337幼苗生理特性的影响

Effects of KNO3 on physiological characteristics of T337seedlings under NaCl stress

  • 摘要: 【目的】研究100 mmol/L NaCl胁迫下不同浓度KNO3对T337幼苗生理特性的影响。【方法】以盆栽2年生苹果砧木T337为试验材料,本试验设置6个处理,即CK1(浇灌清水)、盐胁迫(CK2)以及盐胁迫+喷施4种浓度KNO30.1(T1)、0.2(T2)、0.3 (T3)、0.4 mg/L(T4),测定各处理叶片的叶绿素含量、光合荧光参数、抗氧化酶活性及渗透调节物质的含量,并利用主成分分析对其进行综合评价。【结果】随盐胁迫(CK2)时间的延长,T337叶片光合色素(Chl a、Chlb、Chla/b、Chla+b),气孔导度(Gs)、蒸腾速率(Tr)、净光合速率(P_n)、PSⅡ最大光化学效率(Fv/Fm)、光化学淬灭系数(qP)含量均呈逐渐下降的趋势,非光化学淬灭系数(qN)、初始荧光(F_o)、胞间二氧化碳浓度(Ci)、相对电导率(REC)、丙二醛(MDA)含量、脯氨酸(Pro)均呈上升趋势,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)呈先升后降的趋势。与CK1相比,盐胁迫+外源KNO3(T1-T4)处理后T337幼苗叶片各指标均呈现不同幅度变化,且存在明显的浓度效应,并以T3(0.3 mg/L KNO3)处理叶片叶绿素含量、Gs、Tr、P_n、Fv/Fm、qP降幅最小,Ci、REC、MDA、qN、F_o升幅最小,Pro及SOD、POD、CAT升幅最大。综合评价表明,各处理对T337幼苗特性的效应依次为:CK1>T3>T1>T2>T4>CK2。【结论】综上,施用适宜浓度外源KNO3可有效改善盐胁迫下T337幼苗光合能力,显著提高抗氧化酶活性和渗透调节物质含量,降低细胞膜透性,从而达到缓解盐胁迫的作用,且以0.3 mg/L KNO3处理缓解盐胁迫对T337造成的伤害效果最好。

     

    Abstract: 【Objective】To investigate the effects of different concentrations of KNO3 on the physiological characteristics of T337 seedlings under 100 mmol/L NaCl stress.【Method】Using a potted 2-year-old apple rootstock T337,as the test material,six treatments were established in this experiment,namely CK1(fresh water irrigation),salt stress (CK2),and salt stress+spraying with four concentrations of KNO30.1 (T1),0.2 (T2),0.3 (T3),and 0.4mg/L(T4),and the chlorophyll content,photosynthetic fluorescence parameters,antioxidant enzyme activities and osmoregulatory substances of the leaves of each treatment were measured and evaluated using the chlorophyll content,photosynthetic fluorescence parameters,antioxidant enzyme activities,and osmoregulatory substances of each treatment were determined and comprehensively evaluated using principal component analysis.【Result】Photosynthetic pigments (Chl a,Chl b,Chl a/b,Chl a+b) in T337 leaves increased with increasing time under salt stress (CK2).Stomatal conductance (Gs),transpiration rate (Tr),net photosynthetic rate (Pn),maximum photochemical efficiency of PSⅡ(Fv/Fm),and photochemical quenching coefficient (qP) contents all showed a decreasing trend.Nonphotochemical quenching coefficient (qN),initial fluorescence (Fo),intercellular carbon dioxide concentration (Ci),relative conductivity (REC),malondialdehyde (MDA) content,and proline (Pro) showed an increasing trend,while superoxide dismutase (SOD),peroxidase (POD),and catalase (CAT) activities first showed an increasing trend and then a decreasing trend.Compared with CK1,all indicators of T337 seedlings showed different magnitude of changes after salt stress+exogenous KNO3 treatment (T1-T4),and there were obvious concentration effects,and the chlorophyll content,Gs,Tr,Pn,Fv/Fm,qP decreased the least in T3 (0.3 mg/L KNO3)-treated leaves,Ci,REC,MDA,qN,Fo.The comprehensive evaluation showed that the effects of each treatment on the characteristics of T337 seedlings were in the following order:CK1>T3>T1>T2>T4>CK2.The best effect was achieved by 0.3 mg/L KNO3 treatment to alleviate the damage caused by salt stress to T337.【Conclusion】The application of exogenous KNO3 at appropriate concentrations can effectively improve the light and ability of T337 seedlings under salt stress,significantly increase the antioxidant enzyme activities and osmoregulatory substances,and reduce cell membrane permeability,thus achieving the effect of alleviating salt stress,and the best effect of 0.3 mg/L KNO3 treatment to alleviate the damage caused by salt stress on T337.

     

/

返回文章
返回