Abstract: 【Objective】 To study the seed germination characteristics of Mandragora chinghaiensis.【Method】 The seeds of Mandragora chinghaiensis were used as the test material, and three methods of germination were selected: conventional sowing,low temperature treatment（4 ℃） and tissue culture.【Result】 The viability of M. chinghaiensis seeds was 65% by TTC method staining. Conventional sowing and low temperature treatment failed to germinate the seeds of M. chinghaiensis, but only the seedlings were obtained by tissue culture. The most suitable pre-treatment for seeds was 300 mg/L of GA3 soaked for 16 h.The best disinfection method was 20% NaClO disinfection 15 min. The most suitable base medium for germination is 1/2MS. The most favorable hormone combination for seed germination of M. chinghaiensis was 1/2 MS+6-BA 2 mg/L+NAA 0. 1 mg/L+ GA3 0. 3 mg/L.【Conclusion】 By exploring the germination system of M. chinghaiensis seeds,this study can alleviate the bottleneck problem that M. chinghaiensis seedlings are extremely difficult to obtain for artificial propagation,and provide new support for further research on its pharmacological value and biological properties.