Abstract: 【Objective】 To understand the degree of differentiation,variation and diversity characteristics of Hippophae rhamnoides subsp. sinensis in different populations in Gansu Province.【Method】 Fortynine wild Chinese H. rhamnoides subsp. sinensis resources collected in Gansu Province were systematically analyzed.【Result】 The 14 phenotypic traits of Chinese H. rhamnoides subsp. sinensis showed different degrees of difference among different populations,and the average coefficient of variation was Gannan（19. 35%）＞Yuzhong（18. 24%）＞Tongwei（17. 11%）. There was a significant positive correlation between leaf traits（P＜0. 01）,but no significant correlation with other traits（P＞0. 05）,and the correlation between 1 000 grain weight and branch length was highly significant（P＜0. 01）. Principal component analysis showed that the contribution rate of the source of phenotypic diversity of H. rhamnoides subsp. sinensis in different populations was:branch length ＞ thousand grain weight ＞ leaf length ＞ fruit longitudinal diameter ＞ fruit transverse diameter. Cluster analysis showed that the phenotypic traits of wild H. rhamnoides subsp. sinensis among different populations had a discontinuity in geographical distribution.【Cnclusion】 The three wild Chinese wild H. rhamnoides subsp. sinensis with different populations have rich phenotypic variation and environmental adaptability（CV＞10%）,which have great development potential and utilization value.