Abstract: 【Objective】 Magnetic resonance imaging（MRI） was employed to monitor the real-time alterations in renal serotonin receptors in vivo, presenting a non-invasive imaging technique for the prompt identification of drug-induced acute kidney injury.【Method】 A magnetic resonance contrast agent（HTGd） engineered with serotonin receptor-targeting capabilities was developed based on the serotonin group.By analyzing the T1-weighted magnetic resonance imaging（T1WI） signals of serotonin receptors in mouse kidneys, an early MRI detection approach was investigated for the identification of drug-induced kidney injury.【Result】 In vitro endocytosis experiments revealed that the uptake of HT-Gd by renal tubular epithelial cells（HK-2） was 36. 8 pg/cell following a 12-hour cisplatin pretreatment, contrasting with a mere 14. 7 pg/cell uptake in cells devoid of cisplatin treatment. In vivo magnetic resonance imaging studies demonstrated that HT-Gd heightened the renal T1WI signal-to-noise ratio to 40. 5% in mice subjected to cisplatin treatment for 24 hours, approximately double that of mice not treated with cisplatin（SNR=20. 2%）.【Conclusion】 The visualization of serotonin receptors in the kidney using HT-Gd has enabled the early diagnosis of cisplatin-induced acute kidney injury, offering a novel reference technique for the early diagnosis and treatment exploration of drug-induced acute kidney injury.