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沃金黑牛AIDA基因第10外显子多态性检测及关联分析

Polymorphism detection and association analysis of exon 10 of AIDA gene in Wojin Black cattle

  • 摘要: 为探索Axin互作背部化相关基因(Axin interactor dorsalization associated, AIDA)多态性对沃金黑牛生长及肉质性状的影响,试验以62头12月龄健康沃金黑牛为研究对象,采用Sanger测序方法研究AIDA基因的多态性并对AIDA基因第10外显子不同基因型与生产数据进行关联分析。结果表明:沃金黑牛的AIDA基因第10外显子第89 bp处存在A/C突变,且为错义突变,氨基酸由赖氨酸突变为谷氨酰胺。突变存在3种基因型,分别为AA、CA和CC,CA为优势基因型,且A为优势等位基因。该位点属于中度多态(PIC=0.335),处于哈代-温伯格平衡状态(P>0.05)。关联分析结果表明,该位点的多态性与雄性牛背膘厚呈显著相关(P<0.05),但与沃金黑牛体高、十字部高、体斜长、胸围、腹围、管围、眼肌面积均无显著关联(P>0.05)。

     

    Abstract: To explore the influences of AIDA genes on the growth and meat quality traits of Wojin Black cattle, 62 healthy Wojin Black cattle at 1 year old were selected as the study subjects. Sanger sequencing method was used to study the polymorphism of AIDA gene and to analyze the association between different genotypes of AIDA gene exon 10 and production data. The results showed that a missense A/C mutation at the 89 th bp of exon 10 of AIDA gene was detected resulting in a change from lysine to glutamine. Among the three genotypes of being AA, CA and CC, CA was the dominant genotype with A of being the dominant allele. This locus was in Hardy Weinberg equilibrium(P>0.05) and in moderate polymorphism(PIC=0. 335). The association analysis showed that there were significant associations between the variations of AIDA gene and the backfat thickness of male cattle(P<0.05), and no associations were found for body height, cross height, oblique length, chest circumference, abdominal circumference, cannon circumference and eye muscle area of Wojin Black cattle(P>0.05).

     

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