Abstract: Myostatin, a member of the transforming growth factor-β（TGF-β） superfamily, is a negative regulator of skeletal muscle growth. Inactivating mutations in the Myostatin gene would result in a hyper-muscularity phenotype known as “double muscling” in the livestock showing the abnormal muscle growth in the fore and hind limbs, shoulders, and hips. With its great potential of being a molecular target for livestock breeding, the Myostatin and its regulation are becoming one of the hot spots for association analysis of growth and meat traits in the animal breeding. In recent years, some advances were made in improving meat production for animals like pigs, cattle and sheep by using gene editing technology to modify the Myostatin gene. This paper reviewed the Myostatin gene and its editing examples in pigs, cattle and sheep to provide a reference for future Myostatin gene editing in livestock.