Abstract: To explore the effect of PAD class on Wisdom Tree Platform in Biostatistics teaching, animal science students of two classes in grade 2020 were randomly divided into the PAD learning group（n=44）and the traditional teaching model group（n=42）. The teaching efficiency would be assessed through/with teaching process, teaching effect, unit testing,questionnaire survey and final exam in one semester. The results suggested that the teaching satisfaction, independent learning ability, group cooperation ability, learning time arrangement, interest in class, combination of theory and practice and knowledge application were significantly higher than those of traditional teaching mode（P＜0.05）. It could be concluded that the PAD class teaching model on Wisdom Tree Platform has promotional value for its advantages of enhancing students′ self-learning ability, optimizing teaching links, bringing about personalized learning, stimulating learning enthusiasm, and improving teaching efficiency.