NCG对热应激大骨鸡精液品质影响的研究

李佳慧; 张焕琪; 施佐; 马巍; 王春强

doi:10.19848/j.cnki.ISSN1005-2739.2023.10.0009

NCG对热应激大骨鸡精液品质影响的研究

锦州医科大学畜牧兽医学院

基金项目:

辽宁省科技厅科技特派项目(2022JH5/10400133)

详细信息

作者简介:
李佳慧(1999—),女,硕士研究生,研究方向为动物遗传与繁育,2421222406@qq.com

通讯作者:
王春强(1980—),男,教授,博士,硕士生导师,研究方向为动物遗传与繁育,153373682@qq.com

中图分类号: S831.5
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出版历程
- 收稿日期: 2023-10-06
- 刊出日期: 2024-01-14

The impact of NCG supplementation on semen quality of large-bone chickens under heat stress

College of Animal Science and Veterinary Medicine, Jinzhou Medical University

摘要

摘要: 试验旨在探究热应激条件下日粮中添加不同浓度N-氨甲酰谷氨酸(NCG)对大骨鸡精液品质的影响。选取48只25周龄大骨鸡，随机分为4组，每组12只鸡，对照组饲喂基础日粮，N1、N2、N3组分别在基础日粮中添加0.08%、0.12%、0.16%NCG,每周对大骨鸡精液运动性能、活率、畸形率及抗氧化指标进行检测，试验期共计90 d。结果表明：与对照组相比，试验组精子活力、质膜完整性与直线速率等显著提高(P<0.05),摆动性、鞭打频率差异不显著(P>0.05),精子畸形率显著下降(P<0.05);N1组超氧化物歧化酶(SOD)活性最低，N3组最高，N1组与N3组间显著差异(P<0.05);各组间过氧化氢酶(CAT)活性、丙二醛(MDA)含量均无显著差异(P>0.05)。说明日粮中添加不同水平NCG降低了热应激对大骨鸡精液品质造成的不良反应，其中N3组效果最佳。
- NCG /
- 热应激 /
- 大骨鸡 /
- 精液品质 /
- 抗氧化指标
Abstract: The aim of this study was to investigate the effects of dietary supplementation of different concentrations of N-carbamylglutamic acid（NCG） on semen quality of large-bone chickens under heat stress. Forty-eight 25-week-old large-bone chickens were randomly divided into four groups, in each of which was 12 chickens. The control group was fed the basal diet, and the experimental groups（N1, N2 and N3） were fed the basal diets supplemented with 0.08%, 0.12% and 0.16% NCG, respectively. The motility, viability, malformation rate and antioxidant indexes of semen of big-bone chickens were tested weekly for a total of 90 d. The results showed as follows: compared with the control group, the semen vitality, progression and plasma membrane integrity were significantly increased（P＜0.05）, the rates of sperm malformation were significantly decreased（P＜0.05）, while the oscillating and whipping frequency showed no significant difference（P＞0.05） in 3 experimental groups. The superoxide dismutase（SOD） levels in group N3 were significantly higher than those in group N1（P＜0.05）,and there was no significant difference（P＞0.05） for CAT activity and the content of MDA among the groups. The study indicated that dietary supplementation of NCG could reduce the detrimental impact of heat stress to the semen quality of large-bone chickens and the supplementation of 0.16% NCG could produce the best mitigation effect.
- NCG /
- heat stress /
- large-bone chicken /
- semen quality /
- antioxidant index

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