Abstract: The construction of new agricultural science majors in higher education institutions aims to cultivate new agricultural science-technology talents who understand and love agriculture. The integration of industry and education plays an important role in education and teaching, talent cultivation, industrial development, and innovation and entrepreneurship. Animal Reproduction, one of the basic courses of animal science major, mainly studies the laws and techniques of animal reproduction, plays an important role in professional talent cultivation by preparing students with solid foundation for learning specific subjects, with the characteristic of fast content updates and strong practicality. The article takes the national first-class course, Animal Reproduction, at Nanjing Agricultural University as an example, to expound the necessity of integrating industry and education, the practical effects of resetting teaching objectives, rebuilding teaching systems, and optimizing assessment methods with further reflection on the employed improvement methods, to provide reference for the construction of professional courses in higher agricultural colleges in China.