Abstract: The aim of this experiment was to study the effect of cold stress on the expression of antioxidant-related genes in muscles of Min pigs. Nine Min piglets at 1-2 months were randomly divided into three groups, which were fed at room temperature（22～25 ℃）, short-term cold exposure（4 ℃ for 3 d） and long-term cold exposure（4 ℃ for 15 d）, respectively. The mRNA expression levels of superoxide dismutase（SOD）, glutathione peroxidase（GPX）, Nuclear factor erythroid 2-related factor 2（Nrf2） and Kelch-like ECH-associated protein1（Keap1） in longissimus dorsi muscle of piglets in room temperature group, short term cold exposure group and long term cold exposure group were detected by quantitative real-time PCR. The results showed as follows: Compared with the room temperature control group, no significant difference in the expression of the SOD gene. However, the significant increase in GPX expression（P＜0.05） was detected in the short-term cold exposure group and the long-term cold exposure group. The expression level of Nrf2 gene was significantly increased in the short-term cold exposure group（P＜0.05） and decreased in the long-term cold exposure group. There was no significant difference in Keap1 gene expression between short-term cold exposure group and room temperature group, but it was significantly decreased in long-term cold exposure group（P＜0.05）. The results indicated that cold stress could impact the expression of antioxidant-related genes in muscles of Min pigs.