Abstract: In order to study the factors affecting the effect of superovulation of donors and the pregnancy rate of recipients in embryo transplantation of sheep, two sheep breeds, Suffolk sheep and East fryson sheep, were treated as donors, and local Altay sheep breeds were as recipients. The five factors including the batches of follicle-stimulating hormone（FSH）, long-distance transportation, insemination methods, technician’s skills and recipients’ synchronization time were studied in the experiment. The results showed as follows: no significant difference was found for the effect of the batches of FSH on the available embryo rate of Suffolk and East fryson sheep（P＞0.05）. A significant decrease in the embryo number and fertilization rate of the donor sheep, both Suffolk sheep and East fryson sheep after long-distance transportation, compared with that of the control group（P＜0.01）. The insemination method of uterine horn insemination + conventional insemination could produce a higher recovery rate of embryos than that of either conventional or horn fertilization（P＜0.01）, respectively, in which the recovery rate of uterine horn insemination was significantly higher than that of conventional insemination method（P＜0.05）. The pregnancy rate of recipients group by skilled operators significantly higher than that of control group（P＜0.05）. No significant difference in both estrus rate and conception rate between the recipients was found with ±12-hours difference in onsets of estrus by different device removal time（P＞0.05）. The results of the experiment could provide a reference for scientific researchers and technicians engaged in embryo transfer work.