Abstract: Bioinformatics is an interdisciplinary field of science that involves using computer technology to collect, store, analyze and disseminate biological data and information. With the wide application of bioinformatics in the field of animal husbandry, the importance of the teaching of Bioinformatics course in the major of animal science has addressed increasingly. Based on the teaching practice of Bioinformatics course at the College of Animal Science and Veterinary Medicine of Henan Institute of Science and Technology, this paper proposed some teaching methods of improving the teaching effects of Bioinformatics, which were as follows: the combination of off-line teaching and on-line learning, balancing of theory and practice teaching, cultivation of students’ hands-on abilities, introduction of employment-oriented teaching, integration of scientific findings into teaching and feeding back scientific research through teaching. The application of these teaching methods mentioned above proved to be helpful in improving the teaching effectiveness of Bioinformatics course, inspiring students’ interest in learning, enhancing the practical application ability of students majoring in animal science.