不同钝化剂对镉污染下涪城麦冬叶片生理特性的影响
Effects of Different Passivating Agents on Leaf Physiological Characteristics of Fucheng Ophiopogon japonicus Under Cadmium Pollution
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摘要: 为探讨不同钝化剂对镉污染下涪城麦冬的缓解作用,以涪城麦冬为材料,采用盆栽法,设置镉污染土壤中(镉含量1.19mg·kg-1)混施秸秆生物炭(Br)、粉煤灰(Fh)、汉白玉(Ar)、硅藻土(Dm)和菌渣(Me)的5个处理,以不添加任何钝化剂的当地镉污染土壤作为对照,观察钝化剂对涪城麦冬叶片生长状况的影响,并检测叶片细胞中叶绿素含量、丙二醛含量、保护酶活性、可溶性蛋白和可溶性糖含量。结果表明:用钝化剂秸秆生物炭处理的麦冬叶片生长更好,其叶绿素、可溶性蛋白、可溶性糖含量均比对照有不同程度的提高,秸秆生物炭能显著提高麦冬叶片细胞的过氧化氢酶(CAT)活性,但超氧化酶歧化酶(SOD)和过氧化物酶(POD)活性并未受到影响。除菌渣外,其他4种钝化剂均能缓解丙二醛(MDA)含量在镉污染下的增加。说明施加秸秆生物炭有效提高了镉污染下麦冬叶片细胞的抗氧化能力,缓解了镉污染对麦冬叶片细胞带来的伤害。Abstract: In order to explore the mitigation effects of different passivating agents on Fucheng Ophiopogon japonicumcadmium pollution,method of pot planting Fucheng Ophiopogon japonicus were used.Polluted soils were mixed with straw biochar in the selected farmland soil(Br),fly ash(Fh),white marble(Ar),diatomaceous earth(Dm)and mushroom residue(Me)as experimental groups,and local polluted soil without adding any passivation agent as a control group for planting it.The effect of passivating agent on the leaf growth of Fucheng Ophiopogon japonicus were observed and the content of chlorophyll,malonaldehyde(MDA)content,protective enzyme activity,soluble protein and soluble sugar content were determined.The results showed that the leaf length,which treated with straw biochar,was longer than that of the control group.The catalase(CAT)activity of Ophiopogon japonicus leaf cells was significantly increased by straw biochar.However,the activities of superoxide dismutase(SOD)and peroxidase(POD)of Ophiopogon japonicus leaf cells treated with passivating agent were not increased.Except for the bacterial residue,the other four passivating agents could alleviate the increase of MDA content under cadmium pollution.Treated with the straw biochar increased the antioxidant level of Ophiopogon japonicus leaves,mitigated the harm of cadmium pollution to Fucheng Ophiopogon japonicus.