Abstract: Mushroom polysaccharide with a variety of activities,but its complex structure,extraction and analysis method is various and each has its own characteristics.In order to promote the industrial production of edible fungi polysaccharides,this paper mainly introduced the advantages and disadvantages of the extraction,separation,purification and structure detection methods of edible fungi polysaccharides in recent five years,as well as the mechanism of biological activity of edible fungi polysaccharides.It was concluded that although there were many analytical methods of polysaccharides from mushroon,there were few methods that could be applied to the extraction of polysaccharides from industrial production due to its complexity.Therefore,efforts should be made to improve the extraction rate and purity without destroying the structure of polysaccharides.