Abstract: Under the background of the new era, the construction of “innovation and entrepreneurship” education practice base in applied colleges and universities has far-reaching significance. The practice base not only promotes the improvement of the practice teaching system, but also strengthens the construction of a high-level practice teacher team and improves the hardware facilities of the practice base by creating a practice base model of “integration of production and education”. In addition, the promotion of the popularity of the practice base, the ability of scientific research and innovation and the optimization of the management mechanism of the practice base are also important aspects to promote its development. These measures jointly build the development path of the “innovation and entrepreneurship” education and practice base of traditional Chinese medicine in the new era, which plays an important role in promoting the training of high-quality applied technology professionals with “excellent innovation” and the construction of first-class majors.