Abstract: In order to promote rapid cutting seeding of Tetrastigma hemsleyanum. The split plot experiment method was used to study the number of stem nodes and shading rate of cuttings for Tetrastigma hemsleyanum. The survival rate, length of new shoots, number of leaflets and number of roots for Tetrastigma hemsleyanum under different treatments were compared and analyzed.The results showed that the correlation coefficients between length of new shoots, number of leaflets and survival rate were 0.824 0 and 0.774 5, respectively. Two stem nodes（A2） and 60% shading（B2）, the survival rate（91.33%）, new shoots length（16.11 cm） and number of leaflets（4.3） were higher than other combinations. It is believed that the cuttings should have two stem nodes and 60% shading treatment in the process of trial have the best results.