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癸酸钠抗性菌株产达托霉素的发酵补料策略

Fermentation Feed Strategy for Daptomycin Production by Sodium Caprate-resistant Strains

  • 摘要: 为提高达托霉素产生菌玫瑰孢链霉菌的发酵能力,通过筛选癸酸钠抗性菌株,优化其发酵瓶补加正癸酸的方法,提高达托霉素的摇瓶效价值。首先对原始菌株进行癸酸钠抗性选育,筛选出一株空白摇瓶效价值为43.4 mg·L-1的菌株TR21-29,利用该菌株对癸酸盐的耐受性,验证其发酵瓶补加正癸酸的最优方式。主要考察正癸酸的耦合剂,向发酵瓶添加正癸酸的补料时间、补料浓度和补料间隔,确定发酵过程中补加正癸酸的关键参数。结果表明:补料可将抗性菌株TR21-29的原始效价值由43.4 mg·L-1提高至138.1 mg·L-1,提高率为218.2%。补料方式为配制0.5 g·mL-1的正癸酸溶液,在发酵瓶培养24 h后开始补加,补料终浓度为1.5 mmol·L-1,补料间隔为4 h。

     

    Abstract: In order to improve the fermentation ability of daptomycin-producing bacterium streptomyces roseolae, by screening sodium caprate resistant strains, optimizing the method of supplementing N-decanoic acid in its fermentation flask, improving the shake flask effect value of tropicolin.The original strain was selected for sodium caprate resistance, a strain TR21-29 with a blank shake flask efficacy value of 43.4 mg·L-1 was screened out.We took advantage of the strain′s tolerance to caprate, verified the optimal strategy of supplementing N-decanoic acid in its fermentation flask.This study mainly investigated the coupling agent of N-decanoic acid, and feed time, feed concentration and feed interval for adding N-decanoic acid to fermentation flasks, determining the key parameters for the addition of N-decanoic acid to fermentation flasks.The results showed that the original efficacy value of the resistant strain TR21-29 was increased from 43.4 mg·L-1 to 138.1 mg·L-1,and the improvement rate was 218.2%.The feeding method is to start adding N-decanoic acid solution after culturing in the fermentation flask for 24 hours.The concentration of N-decanoic acid is 0.5 g·L-1,the feed final concentration is 1.5 mmol·L-1,and the feed is fed every 4 hours.

     

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