Abstract: Based on the concept of OBE,it aims to cultivate application-oriented talents, emphasizing the foundation emphasizing the cultivation of students′ multi-dimensional ability. This paper discusses the exploration and practice of the mixed teaching mode of biochemistry course in applied undergraduate university, and elaborates the online and offline curriculum design how to combine online and offline, concrete implementation, assessment and evaluation. Based on the OBE concept, a new blended teaching mode was constructed, student-oriented, course-based blended teaching greatly improves the learning effect of students′ independence, paying attention to cultivating students′ self-learning ability, summary ability, expression ability, cooperation ability.It had played a reference and demonstration role in the cultivation of application-oriented talents in schools, guided by social needs, and cultivates high-quality application-oriented innovative talents.