Abstract: In order to promote the cultivation of a new Camelina sativa variety‘Jilan No.1’.This experiment compared the agronomic performance of ‘Jilan No.1’ at three different planting row spacing（15, 30 and 45 cm） in western part of China（Ili Autonomous Pefectures of Xinjiang） and the eastern part of China（Lianyungang of Jiangsu Province） from March to June. The results showed that‘Jilan No.1’ had more potential for high yield in Xinjiang Region.The harvest yield and oil content of Camelina sativa in both regions were higher, the seed yields was 204.56 and 187.58 g·m^-2, oil contents reached 43.04% and 42.07%, the monounsaturated fatty acid contents were 22.02%-26.07% and 22.28%-26.46%, and the polyunsaturated fatty acid contents were 50.19%-57.90% and 51.44%-57.58%, respectively. The planting row spacing of 30 cm in both areas significantly increased the yield and linolenic acid content of ‘JilanNo.1’. And the highest seed yield was obtained at 30 cm planting row spacing in Xinjiang. In conclusion, ‘Jilan No.1’ is more suitable for planting in Xinjiang with a suitable row spacing of 30 cm.