Abstract: In order to promote sex induction of industrial hemp,reduce the cost of field work and facilitate the combination between lines.In this experiment,industrial hemp Longma 6 was used as experimental materials to investigate the effects of two exogenous agents of AgNO₃ and gibberellin（GA₃）on sex differentiation and pollen viability of industrial hemp.The results showed that AgNO₃ and GA₃ had male inducing effect on industrial hemp,and could significantly reduce the proportion of famale-male ratio.It was the best effect of male induction to spray 0.40 mmol·L^-1 AgNO₃ at 80 days after sowing.It was the best effect of male induction to spray 200 mg·L^-1 GA₃ at 60 days after sowing.