Abstract: In order to promote the yield of kiwifruit and further accelerate the industrial development, kiwifruit Guichang was used as experimental material, pollinated by bees and artificial methods, and the effects of different pollination methods on production indexes, fruit morphology and fruit quality were measured.The results showed that compared with the optimized artificial pollination method, Oriental bee pollination significantly improved the fruit setting rate and seed setting rate, significantly increased the longitudinal diameter of fruit, and highlighted the long cylindrical fruit shape of Guichang kiwifruit; In terms of fruit quality, the titratable acid content of kiwifruit increased significantly after pollination by Oriental bee.It showed that Oriental bee pollination could significantly increase the yield of kiwifruit, improve the appearance of kiwifruit and save human investment in pollination.