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不同产地黑木耳多糖的抗氧化活性比较

Comparison of Antioxidant Activities of Auricularia auricular Polysaccharides from Different Producing Areas

  • 摘要: 为促进通江黑木耳的进一步开发利用,以东北小碗耳、通江黑木耳、白背黑木耳3种不同产地的黑木耳为试材,以提取率和多糖含量为评价指标,利用热水提取法、Sevag脱蛋白得到3种黑木耳多糖,进行Molish反应分析,采用体外测试法考察不同产地黑木耳多糖的抗氧化活性。结果表明,东北小碗耳的多糖提取率和多糖含量最高,分别为2.48%和43.64%,意味着东北小碗耳的多糖提取率和多糖含量存在优势。不同产地黑木耳的多糖有不同的抗氧化活性,其中通江黑木耳多糖在抗氧化活性方面存在较为明显的优势,羟基自由基,1,1-二苯基-2-苦味基肼(DPPH·)自由基,2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS+·)自由基的清除率分别高达50.11%,56.68%和32.64%,还原力能力高达0.12,表明通江黑木耳多糖抗氧化活性方面更具有潜力。

     

    Abstract: In order to select and obtain the high quality Ganoderma lucidum strains suitable for cultivation in Xizang, the main agronomic characters and active components in fruit bodies of 6 different Ganoderma lucidum strains were compared and analyzed by using bag material cultivation. The results showed that strains 15 and 26 were all Ganoderma lucidum. The average single weight of fruiting body of strain 26 was the highest(82.01 g), the diameter of cap was the largest(16.00 cm), and the biological efficiency was the highest(23.60%). The content of triterpenoids in fruitus of strain 15 was the highest(1.91%), which was 282.00% higher than that in pharmacopoeia(1.88%). The content of polysaccharides in fruitus of Ganoderma lucidum strain 15 was the highest(1.27%), which was the highest. The polysaccharide and triterpene contents of isolates 15 and 26 were significantly higher than those of other Ganoderma lucidum strains. According to the main agronomic traits, biological efficiency and active ingredient contents, Ganoderma Lucidum 15 and 26 were suitable for cultivation and promotion in Lhasa area.

     

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