Abstract: Under the background of the rapid development of information technology, online education based on the Internet has brought new opportunities and challenges to the reform of education and the construction of teaching systems. The combination of online and offline teaching mode is the complementary and integration of network teaching and traditional classroom teaching. This article takes Soil Fertilizer Science, a professional basic course at Heilongjiang Bayi Agricultural University, as an example. Relying on the Chaoxing Learning Platform, an online and offline mixed teaching model is constructed to achieve pre-class preview, in-class interaction, post-class review, experimental operation, and all-round assessment and evaluation. The organic combination of multiple teaching links not only effectively improves students’ learning initiative and enthusiasm, but also emphasizes the importance of process assessment. The new teaching model effectively promotes the deep integration of online education and traditional education, further explores and strengthens course content, emphasizes the application of knowledge, and cultivates agricultural applied talents with a solid theoretical foundation and innovative ability for the construction of modern agriculture in the new era.