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老化处理对不同活力玉米种子生理特性的影响

Effects of Aging Treatment on Physiological Characteristics of Maize Seeds with Different Vigor

  • 摘要: 为研究老化种子中生理指标与种子活力的相关性,本研究采用高温高湿人工加速老化的方法处理3份不同活力玉米杂交种,测定在不同老化处理下种子的吸水特性、种子外渗物质、ROS、重要酶类及调节物质等生理生化指标的变化。结果表明:随着老化程度加深,种子的吸水能力在吸胀12~24 h受抑制逐渐明显。活力强的种子氨基酸外渗量低,·OH清除速率和H2O2含量随老化时间延长呈下降趋势,不同活力种子间差异不显著。随老化时间延长,种胚内的可溶性糖、Pro含量、DHA活性及POD活性出现不同程度下降,活力强的种子仍能保持较高调节物质含量及酶活性。DHA活性、POD活性及Pro含量与种子活力显著相关,说明可通过测定这些生理指标判定种子的活力水平及耐贮性。

     

    Abstract: In order to explore the performance rules of the new maize varieties traits in different analysis models, and make objective and reasonable comprehensive evaluation, this paper used AMMI model, grey correlation degree and principal component analysis to analyze the 14 traits of 15 maize varieties(lines).The results showed that the interaction effect of the principal components IPCA1,IPCA2 and IPCA3 of the interaction between maize yield genotype and environment accounted for 90.49%,indicating that AMMI model analyzed the interaction information between genotype and environment thoroughly; In the analysis of variety stability Di and pilot discrimination parameter Dj,varieties G1 and G3 belonged to high and stable yield varieties, G15 was low and stable yield varieties, and pilot E2,E1 and E5 had strong discrimination to varieties; Through the interaction of varieties and locations, it was concluded that varieties G1,G3 and G15 had good adaptability and stability.In conclusion, AMMI analysis scientifically reflected the interaction effect of additive genetic model in combination with the field yield performance and significant difference of varieties.The grey correlation degree and principal component analysis showed that the grey comprehensive evaluation values Gi were 0.757 4 and 0.716 0 respectively.Varieties G1 and G3 with comprehensive scores of principal components of 0.62 and 0.58 had good comprehensive characters and reliable high yield, stability and adaptability.G15 ranked low in each analysis method, indicating that its comprehensive character was poor, and it was a low yield and stable yield variety.

     

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