Abstract: In order to effectively alleviate the problems of high production cost, difficult labor and low production efficiency of rice in cold areas, promote cost saving, efficiency increase and sustainable agricultural development. The experiment took Kenjing 8 as the test variety, and a single factor randomized block design was adopted: sowing amount 4 level（B1, B2, B3 and B4 dry seed amount 100, 200, 250 and 300 g per plate, respectively）, and conventional treatment（CK, 100 g per plate）. The effects of different seeding densities on rice emergence rate, seedling quality, α-amylase activity and antioxidant enzyme activity were studied. The results showed that under the “double immunity” mode of no soaking seed and no spurting, the emergence rate, seedling formation rate and endosperm residue of rice seedling were significantly increased. Compared with CK treatment, B3 treatment increased by 10.77%（emergence rate）, 13.16%（seedling formation rate） and 46.19%（endosperm residue） in 2 years. However, with the increase of seeding amount, the stem base width, root number, the distance between the first ear and the dry matter mass of the above ground decreased. The activity of α-amylase in seedling stage was significantly higher than that in conventional control at thoracolapril-injection stage, and significantly lower at 1.1 leaf stage-2.1 leaf stage under double immunity mode. The α-amylase activity decreased first and then increased with the increase of seeding density, and decreased gradually with the germination and growth of seeds. The activity of antioxidant enzymes in rice leaves in the “double immunity” mode was lower than that in the conventional control, and the trends of superoxide dismutase and catalase showed a decreasing trend with the increase of seeding density.