Abstract: In order to study the molecular mechanism of the formation of Cymbidium ‘Xiaofeng’mutant,using shoot tips of test-tube seedlings of Cymbidium ‘Xiaofeng’and its leaf color and pattern mutant as explants,effects of leaf color and pattern mutation on micropropagation characteristics were studied by the use of tissue culture method,and the contents of photosynthesis pigment,soluble sugar and protein in leaf and rhizome were also measured.The results showed that the leaf color and pattern mutation significantly reduced the rhizome induction rate from shoot tip,rhizome proliferation coefficient and seedling differentiation rate,but significantly increased the bud differentiation rate of Cymbidium ‘Xiaofeng’.The height of test-tube seedling of the mutant was significantly lower than that of Cymbidium ‘Xiaofeng’,while plant width,the number of leaf and root were significantly higher than that of Cymbidium ‘Xiaofeng’.Besides,leaf color and pattern mutation significantly reduced the contents of Chla,Chlb,total Chl,Caro,soluble sugar and protein in leaves of Cymbidium‘Xiaofeng’.In conclusion,leaf color and pattern mutation significantly reduced micropropagation efficiency and the content of photosynthesis pigment in leaves of Cymbidium ‘Xiaofeng’.