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水杨酸对硝酸铅胁迫下绿豆种子萌发及幼苗生长的影响

Effects of Salicylic Acid on Seed Germination and Growth of Mung Bean Under Heavy Metal Stress

  • 摘要: 为探讨重金属污染对植物的毒害机制,以市售绿豆种子为试验材料,通过不同浓度硝酸铅溶液处理绿豆种子模拟重金属胁迫,探讨水杨酸对重金属胁迫下绿豆种子萌发及幼苗生长的影响。结果表明:随着铅胁迫浓度的增加,绿豆种子的发芽势、发芽率和根长等生理生化指标逐渐降低,根尖细胞在有丝分裂过程中出现了染色体环、染色体桥和微核等异常现象。150mg·L-1硝酸铅胁迫能够显著抑制绿豆种子萌发和生长。而50mg·L-1的水杨酸对硝酸铅胁迫下的绿豆种子萌发和生长的抑制均有一定的缓解作用,根尖染色体分裂异常情况减弱。可见水杨酸能增加植物的抗逆性来抵抗环境对其生长的迫害。

     

    Abstract: In order to explore the toxic mechanism of heavy metal pollution on plants,in this paper,the seeds of mung bean were treated with different concentrations of lead nitrate solution to simulate heavy metal stress,and the effects of salicylic acid(SA)on the germination and seedling growth of mung bean under heavy metal stress were studied.The results showed that with the increase of the concentration of lead nitrate stress,the physiological and biochemical indexes of mung bean seed,such as germination potential,germination rate and root length,decreased gradually.The abnormal phenomena such as chromosome ring,chromosome bridge and micronucleus appeared in root tip cells during mitosis.150 mg·L-1 lead nitrate could significantly inhibit the germination and growth of mung bean seeds.However,the 50 mg·L-1 SA could alleviate the inhibition of seed germination and growth of mung bean under the stress of lead nitrate,and the abnormal condition of root tip chromosome division was weakened.Salicylic acid could increase the stress resistance of plants to resist the persecution of environment.

     

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