Abstract: In order to further optimize the fertilization technology under integration of water and fertilizer for maize, and to promote the effective transformation and utilization of nitrogen fertilizer, effects of different nitrogen application rate on soil organic nitrogen components and yield were studied. The results showed that with the increase of nitrogen application rate, the content of soil acid hydrolyzed total nitrogen, acid hydrolyzed ammonium nitrogen, acid hydrolyzed amino acid nitrogen, and acid hydrolyzed amino sugar nitrogen showed a trend of first increasing and then decreasing. When the nitrogen application rate was 210 kg·ha^-1（N2 treatment）, content of the above four organic nitrogen components was the highest. Acid hydrolyzed ammonium nitrogen was the key factor affecting maize yield, and its content under N2 treatment was higher than that in N3(252 kg·ha^-1), N4(273 kg·ha^-1), N1(147 kg·ha^-1) and N0(0 kg·ha^-1),which significantly increased by 3.55%, 9.86%, 20.37%, and 170.21%, respectively. Yield significantly increased by 1.58%, 2.97%, 18.43%, and 112.89%, respectively. In summary, N2 treatment(nitrogen application rate is 210 kg·ha^-1) has higher nitrogen supply potential, was a more ideal nitrogen application rate.