Abstract: In order to screen and popularize middle and late maturing potato varieties with high yield, high quality and good disease resistance suitable for the third and fourth accumulated temperature zone in Heilongjiang Province, taking Kexin 13 as the control, self bred potato lines were used as experimental materials, the phenological period, morphological characteristics, field characters, tuber quality and yield were compared and analyzed.The results showed that the six tested lines were middle and late maturing potato lines.In the investigation of yield index, potato disease and potato cracking rate, the best line was HSD4,but its commercial potato rate and dry matter content were relatively low.In the investigation of disease resistance, the tested lines only showed potato early blight in the whole growth season.HSD1,HSD3 and HSD5 lines had good resistance to early blight, but the potato block defect was serious, and the secondary growth, potato cracking rate and hollow rate were high.From the comprehensive consideration and evaluation of disease resistance, yield and tuber quality, HSD4 line had the characteristics of high yield, high quality and good disease resistance.It can be popularized in the third or fourth accumulated temperature zone of Heilongjiang Province.