Abstract: Landscape plant biotechnology is an important required course for landscape architecture major, which plays an important role in cultivating students′ innovative ability, practical ability, ideological and political quality. This article reviewed the problems of the course exist in theory and experimental teaching. In this article, the course teaching of landscape plant biotechnology was taken as an example, the reform of theory teaching and experiment teaching of landscape plant biotechnology were discussed.Taking this course teaching as the starting point, and improving students’ comprehensive quality and innovative ability as the guidance, this paper discussed the reform of theoretical and experimental teaching of garden plant biotechnology. By strengthening the construction of professional teaching materials, updating teaching contents and teaching means, deeply mining high-quality resources, strengthening the ideological and political construction of courses. Based on these improvement method to construct scientific teaching objectives and teaching system specially of garden plant biotechnology and cultivate the professional talents of students meet the school requirements and social needs.