Abstract: The agriculture-related majors in colleges and universities are the main positions for the cultivation of scientific and technological talents in the agricultural industry. Providing innovative and entrepreneurial talents to serve the “three-rural” work and help the rural revitalization is the historical task entrusted to agricultural colleges and universities given by the social and economic development in the New Era. The agriculture-related majors should be based on the local, serve the local, highlight the school-running characteristics in the coordinated development with the local economy, implement the educational policy of “ideological and political guidance, feeling education”, take “serving the local, industrial needs” as the goal, firmly adhere to the school-running philosophy of “professional oriented, strengthen ability”, build the “trinity, multi-wheel drive” innovation ability training mode, and cultivate innovative and entrepreneurial talents that can take root in agricultural and rural areas for rural revitalization. The education of agriculture-related professional innovation and entrepreneurship ability should be closely linked with the needs of rural revitalization and development. In terms of professional knowledge and skills education, we should pay attention to the learning of relevant interdisciplinary knowledge, and cultivate applied talents with both “three-rural” feelings and innovation and entrepreneurship ability.