Abstract: In order to study the effects of different factors on leaf cuttings of four Primulina species, the optimum conditions of leaf cuttings for different species were screened. Cutting experiments of P. ‘Purple Fumes’, P.hiemalis, P.shouchengensis and P.longistyla were carried out on different leaf positions and IBA concentrations.The results showed that the best leaf position for cuttings of four Primulina species was the upper leaf, which was easier to produce the new plant than the lower leaf. The treatment effects of different IBA concentrations on the four Primulina species were different. The best cutting effect can be achieved by immersing the incisions of the upper leaves of P. ‘Purple Fumes’, P.hiemalis, P.shouchengensis and P.longistyla in IBA solution with concentrations of 500, 300, 100 and 500 mg·L^-1 for 15 seconds.