Abstract: Major is the basic teaching unit of undergraduate colleges and universities, and first-class major is the foundation of first-class undergraduate education. Major construction is an important content of the quality and connotation construction of higher education institutions, which is directly related to the improvement of the quality of talent cultivation and the level of university management. Implementing first-class major construction for applied undergraduate universities and horticulture majors based on their positioning and characteristics is an important measure to comprehensively implement the spirit of the education work conference and improve the quality of talent cultivation. Based on the “four fundamentals and four combinations” talent cultivation concept, the horticulture major has deepened the comprehensive reform of the major from various aspects, such as building a three-dimensional education pattern, improving the talent cultivation plan, optimizing the curriculum system, constructing a collaborative education model, promoting classroom teaching reform, expanding the “second classroom”, and promoting the development of the discipline to support major construction. The horticulture major has strengthened the construction of a dual-teacher teaching team, paid attention to the construction of grassroots teaching organization and the quality assurance system of professional teaching, and overall improved the level of professional characteristic and connotation construction.