荔枝酸腐病病原菌的形态学及分子生物学鉴定

Morphological and Molecular Identification of the Pathogen of Litchi Sour Rot

摘要: 为了鉴定荔枝酸腐病的病原,利用组织分离法从新鲜发病的荔枝果实组织中分离并纯化出培养物,再经单孢分离获得3个菌株LCG-1、LCG-2和LCG-3;经形态学特征和rDNA-ITS序列分析,从而对分离菌株进行鉴定。结果表明:3个菌株形态学和分子生物学特性一致,综合鉴定为白地霉(Geotrichum candidum Link&Magazin)。根据柯赫氏法则对3个菌株进行致病性测定,接种果实的致病率均为100%,而对照无症状表现,因而证实分离菌株为荔枝酸腐病的病原菌。

Abstract: In order to confirm the pathogen of litchi acid rot,three isolates,LCG-1,LCG-2,and LCG-3,were isolated and purified from fresh litchi fruit tissues by tissue isolation method,and identified by morphological characteristics and the rDNA-ITS sequences analysis.The results showed that the morphological and molecular biological characteristics of the three strains were consistent,and they were identified as Geotrichum candidum Link ＆ Magazin.According to Koch’s rule,the pathogenicity of the three strains were tested.The results showed that the incidence rates of all inoculated fruits were 100%,while the control showed no symptoms.Therefore,the isolates were identified as the pathogen causing litchi sour rot.