Abstract: The extraction technologies of protein and β-glucan in highland barley and oat mainly include alkaline method,enzymatic method and composite method.This article mainly discussed the alkali method,enzymatic method and physical method and their assisting other extraction methods.It is comprehensively concluded that although alkali method was suitable for extracting plant protein,its high concentration of alkali will affect the plant protein,making its nutrients denatured and destroyed,and will produce toxic substances that damage kidney function.Compared with alkaline process,enzymatic reaction conditions were mild and do not produce toxic substances,but its cost was high.The addition of physical method in the extraction process can not only reduce the extraction cost,but also significantly improve the efficiency of plant protein extraction.