Abstract: In order to promote the development and utilization of traditional Chinese medicine（TCM）saponin,this article using four kinds of Chinese herbal medicine that are rich in saponins of mixed water extraction liquid as fermentation substrate with a total expression of bacillus subtilis protease and pectinase or add 5% glucose to fermentation.The mixed water extraction liquid was tested by single factor method to set different extracting time,temperature,pH,and solid-liquid ratio.The determination of the washing effect of fermentation before and after fermentation substrates,light transmittance and saponin was tested then.The results showed that he extraction conditions of traditional Chinese medicine saponin were 60℃,3 h,pH9.0 and the ratio of solid to liquid 1∶18.The washing effect of the saponin extract directly fermented by bacillus subtilis increased by 8.18%compared with that before fermentation.The washing effect of fermentation broth obtained by adding 5% glucose was positively correlated with the activity of protease and pectinase.The light transmittance of the fermentation broth obtained by the two fermentation methods reached the maximum at 32 h.The saponin was basically stable before and after fermentation.In conclusion,the fermentation liquid of traditional Chinese medicine saponin fermented by bacillus subtilis can be used as the early development of washing liquid.