向日葵HafabZ基因的生物信息学和表达分析
Bioinformatics and Expression Analysis of HafabZ Gene in Sunflower
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摘要: 为进一步了解向日葵HafabZ基因的表达特性及功能,通过数据库检索(NCBI)及荧光定量PCR等方法对向日葵HafabZ编码蛋白进行生物信息学和表达模式分析。结果表明:预测HafabZ编码蛋白为酸性且为亲水性蛋白,二级结构主要为无规则卷曲,预测亚细胞定位于叶绿体;系统进化分析发现,HafabZ与薇甘菊fabZ进化关系最近,序列相似性为83%;HafabZ基因表达分析结果表明,在向日葵"L-1-OL-1"开花后7d,HafabZ在向日葵中表达量从高到低排序为:种子>舌状花>管状花>叶>茎>根。在种子发育不同时期,HafabZ在高、低油酸向日葵材料发育前期种子中表达量均较高,均在开花后17d表达量达到最高,之后均明显下调,在开花后22和27d,高油酸向日葵中表达量明显低于低油酸向日葵,表明这段发育时期HafabZ在高、低油酸向日葵种子中可能存在不同的表达调控机制。Abstract: In order to further understand the expression characteristics and functions of HafabZgene in sunflower,the bioinformatics and expression pattern of HafabZencoded protein in sunflower were analyzed by database retrieval(NCBI)and fluorescence quantitative PCR.The results showed that the protein was predicted to be acidic and hydrophilic,and its secondary structure was mainly irregular curl;phylogenetic analysis showed that HafabZhad the closest evolutionary relationship with Mikania micrantha L.fabZ,and the sequence similarity was 83%.The results of HafabZgene expression analysis showed that the order of HafabZ expression from high to low was seed>tongue floret>tube floret>leaf>stem>root on the 7 th day after flowering of sunflower“L-1-OL-1”.At different stages of seed development,the expression levels of HafabZin high and low oleic acid sunflower materials were higher at the early stage of seed development,and reached the highest level at 17 days after anthesis,and then decreased significantly.At 22 and 27 days after anthesis,the expression levels of HafabZin high oleic acid sunflower materials were significantly lower than that in low oleic acid sunflower materials.These results suggest that there may be different regulation mechanisms of HafabZ expression in high and low oleic acid sunflower seeds during this developmental period.
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